Ozone Science & Research Studies
Peer-reviewed studies, government research & university findings. Click any topic to browse.
99.99%Kill Rates
30+Studies
15+Institutions
25+Yrs Research
Bactericidal Effects of High Airborne Ozone on E. coli & S. aureus
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99.99% kill rates achieved for both E. coli and S. aureus within 10–480 seconds at 300–1500 ppm ozone.99.99%
E. coli · S. aureus
Sporicidal Effect of Ozone on Fungal & Bacterial Spores in Water
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Ozone demonstrated potent sporicidal activity, destroying both fungal and bacterial spores in water.Sporicidal
Spores in water
Effects of Ozone & Negative Air Ions on Microorganisms
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Combining ozone with negative ions enhances microbial destruction beyond ozone alone.Enhanced
Dual-action
Use of Ozone Generators Against COVID-19
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U.S. Army validated ozone generators for rapid decontamination against SARS-CoV-2 in enclosed spaces.Validated
SARS-CoV-2
Ozone Disinfection of SARS-Contaminated Areas
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Ozone effectively decontaminates indoor environments exposed to SARS coronavirus.Effective
SARS surfaces
Pseudoviruses for Coronavirus Disinfection Assessment
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Pseudovirus models confirmed ozone’s efficacy as a coronavirus surface disinfectant.Confirmed
SARS-CoV-2
Persistence of Coronaviruses on Surfaces & Inactivation
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Comprehensive review identifying ozone among biocidal agents that effectively destroy coronaviruses on surfaces.Review
Multiple agents
Practical Method for Using Ozone as a Virus Decontaminating Agent
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Developed practical ozone-based virus decontamination protocols for enclosed spaces.Practical
Virus decon
Effect of Ozone on Common Environmental Fungi
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Ozone gas effectively destroys common building fungi, supporting its use in mold remediation.Effective
Building fungi
Practical Application of Ozone as Anti-Fungal Agent
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Defines ozone concentrations, exposure times, and protocols for effective mold destruction.Practical
Protocols defined
Killing Mold on Grapes — Penicillium & Botrytis cinerea
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Ozone killed conidia of all Penicillium/Botrytis species and controlled gray mold on table grapes.Effective
Penicillium + Botrytis
Deactivation of B. globigii Spores (Anthrax Surrogate)
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Virtual total kill within 30 minutes at 1000 ppm ozone + 70% relative humidity. Electronics survived 10+ hours of exposure.~100%
30 min @ 1000 ppm
Ozone in Production Agriculture & Food Processing
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FDA recognizes ozone as GRAS (Generally Recognized as Safe) for food contact applications.GRAS
FDA-recognized
Ozone to Improve Safety of Fresh Fruits & Vegetables
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Reduces microbial contamination on produce without chemical residues — ideal for organic operations.Significant
Produce safety
Ozone Fumigation in Stored Maize
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Ozone effectively penetrates and fumigates stored corn, killing insects and microorganisms.Effective
Grain storage
Ozone Penetration in Stored Grains
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Ozone disinfects grain while maintaining acceptable chemical composition and processing performance.Effective
Composition safe
Ozone & Poultry Production
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Significantly improves poultry biosecurity, air quality, and pathogen reduction while cutting chemical use.Significant
Biosecurity
Effects of Ozone on the Common Bed Bug
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Ozone penetrates hidden harborage areas where pesticide-resistant bed bugs hide.Effective
Bed bugs
Ozone: New Control Strategy for Stored Grain Pests
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Viable chemical-free alternative to fumigants for controlling insects in stored grain.Effective
Grain insects
Benefits of Ozone Surprise Researchers, Consumers
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Twin City Labs: infectious microorganisms eliminated at just 0.05 ppm. EPA: indoor air 2–70× worse than outdoor.0.05 ppm
Kills microorganisms
Fields of Ozone Applications
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Ozone efficacy documented across water treatment, air purification, food processing, and medical use.Overview
Multi-industry
Hydroperoxides Testing — Katz Analytical Services
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0.01 ppm hydroperoxide = ~177 quadrillion molecules/liter. Pathogens never more than 1 micron from destruction. 50× below safety limits.0.01 ppm
177 quadrillion mol/L
Advanced Oxidation Results 2000–2011
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99%+ kill: H1N1, Bird Flu, MRSA, Norovirus, Strep, Pseudomonas, Listeria, E. coli, B. globigii. Chicago schools saw 20% attendance improvement.99%+
9 major pathogens
Scientific References — Ozone’s Validity as Medical Treatment
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Comprehensive collection of peer-reviewed medical ozone therapy references spanning decades of research.Reference
Bibliography
Virucidal Efficacy of Hydrogen Peroxide Vapour
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VHP achieves significant virucidal activity for whole-room decontamination in healthcare settings.Effective
Room-level
H₂O₂ Nebulization — Surface Disinfection
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Aerosolized hydrogen peroxide effectively decontaminates entire rooms — automated no-touch disinfection.Effective
Automated
VHP Inactivation of Adenovirus
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VHP inactivates adenovirus — one of the hardest viruses to destroy due to its non-enveloped structure.Effective
Non-enveloped virus
VHP Inactivation of Ebola Virus
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VHP effectively inactivates Ebola virus on surfaces — critical for healthcare outbreak response.Effective
Ebola on surfaces
VHP in Hospital Rooms
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VHP reduces healthcare-associated infection risks through whole-room decontamination.Effective
HAI reduction
Low-Cost VHP for PPE Reuse During Pandemics
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Validated protocol for decontaminating and reusing PPE during critical shortage situations.Validated
PPE reprocessing
Sonicated H₂O₂ Virucidal Efficacy (Trophon® EPR)
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Ultrasonic H₂O₂ demonstrated effective virus inactivation using European standardized test methods.Effective
Medical devices
Safety Data Sheet (SDS) — Ozone & Bio3Blaster Equipment
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📋 Includes
Hazard identification, first aid measures, handling/storage guidelines, exposure controls, and physical properties.SDS
Documentation
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Disclaimer: Studies are for educational purposes, representing findings of independent researchers, universities, and government agencies. Bio3Blaster ozone generators are for use in unoccupied spaces only. Rule of 3P: No People, No Plants, No Pets during treatment.